Email Address Please enter a valid email address. Connect With Us. Please Enquire This product is discontinued. Schematic showing DNA to protein expression in 60—90 minutes. GST Pull-Downs. Gel Shift Assays. Characterization of Protein Modifications. These systems are useful for many downstream applications, including protein:protein interactions and protein:DNA interactions.
Video Protocols. You are viewing: L Change Configuration. Certificate of Analysis Search by lot number. Not for Use in Diagnostic Procedures. Storage Conditions. Acids Res. See all citations. L, L L Potassium Chloride 2. A, A, A Let's find the product that meets your needs. Talk to a Scientist. For older versions of R, please refer to the appropriate Bioconductor release.
To view documentation for the version of this package installed in your system, start R and enter:. Follow Installation instructions to use this package in your R session. Support ». Please read the posting guide. Post questions about Bioconductor to one of the following locations:. The aim of the study was to generate the missing basic knowledge allowing us to understand the function of TNTs and the mechanism underlying the transfer.
During two years, extensive efforts were put to challenge three main questions: i which cellular compartment s is are transferred; ii what is the molecular mechanism involved in the transfer and iii what are the cargoes transferred. By looking at the well-known protein markers from different subtypes of cellular compartments, we find a particular, specific subclass of vesicles that are present in cells and are transferred to their neighbors.
The transfer from one cell to another is mediated by nanotubes and we found the key actors that regulate this pathway. In addition, we identified a major cargo that is transported, and that this transport occurs specifically in cancer cells and from cancer cells to the normal cells surrounding them. The results of this study are expected to strongly impact broad research fields such as cell biology and cancer biology.
The transport of protein receptor from tumour cells to fibroblasts is a pioneer finding which shall be taken in consideration in cancer-stroma studies and in particular in anti-cancer therapeutic strategies that target the receptor and which are currently under clinical trials. In addition, an innovant experimental approach was invented to detect the transfer of the protein receptor in a mouse model in vivo, opening the way for many clinical applications, such as the test of new drugs that target cancer-stroma communication, a driving force for cancer progression and yet poorly targeted by available treatments.
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